SENSITIVE DETERMINATION METHOD OF CREATINE PHOSPHOKINASE ISOENZYMES BY BIOLUMlNESCENT LIGHT EMISSION

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概要

• 論文の詳細を見る

• Isoenzymes of creatine phosphokinase (CPK-MM, -MB and -BB) were separated by anion-exchange chromatography, with use of stepwise gradient. The separated isoenzymes were subsequently mixed with the reagents necessary for producing nicotinamide adenine dinucleotide, reduced form (NADH). The produced NADH was measured by flow injection analysis for bioluminescent assay. The lower limit of the detection of NADH was at femtomole level. As the results, the determination of CPK-MB and -BB of low activity without the effects of serum background was possible. The reproducibility was good with the coefficient of variations in the range 2.5 - 3.0 % for CPK-MM, 4.0 - 5.2 % for -MB and 7.O - 8.1 % for -BB.

• 社団法人日本分析化学会の論文
• 1983-12-05

著者

• Hirose Shingo

  Kyoto College Of Pharmacy

• Oda Kayoko

  Kyoto College Of Pharmacy

• YOSHIDA Shigeru

  Kyoto College of Pharmacy

• TAKEDA Tatsumori

  Ueno Civic Hospital

関連論文

• SENSITIVE DETERMINATION METHOD OF CREATINE PHOSPHOKINASE ISOENZYMES BY BIOLUMlNESCENT LIGHT EMISSION
• FLUORESCENCE LABELING OF PSEUDOURIDINE AND 5-FLUOROURACIL FOR HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC SEPARATION

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