FLUORESCENCE LABELING OF PSEUDOURIDINE AND 5-FLUOROURACIL FOR HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC SEPARATION

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概要

• 論文の詳細を見る

• Reaction with 4-bromomethyl-7-methoxycoumarin(Br-Mmc) allowed the fluorescence labeling of authentic pseudouridine (iJjUrd) and 5-fluorouracil(5FU) using 18-crown-6 as a catalyst. The procedure was rapid, simple and requires little or no experience in derivatization techniques. The reversed phase high performance liquid chromatographic(HPLC) separation of ψ Urd-Mmc and 5FU-Mmc esters was studied with a C_<18> column. Determination limits obtained for ψUrd and 5FU with a new f luorometric detection were 100 pg and 10 pg per 1 Ul injection volume, respectively. Linear calibration plots for peak height versus concentration were up to 10 ng per injection. This fluorescence labeling method for HPLC has an importance in the accurate determination of ψUrd and 5FU which are the most reliable tumor marker and pyrimidine antimetabolite, respectively.

• 社団法人日本分析化学会の論文
• 1984-06-05

著者

• Hirose Shingo

  Kyoto College Of Pharmacy

• Hirose Shingo

  Kyoto Pharmaceutical University

• Iwamoto Masaki

  Kyoto Pharmaceutical University

• YOSHIDA Shigeru

  Kyoto College of Pharmacy

• YOSHIDA^[○!R] Shigeru

  Kyoto Pharmaceutical University

関連論文

• SENSITIVE DETERMINATION METHOD OF CREATINE PHOSPHOKINASE ISOENZYMES BY BIOLUMlNESCENT LIGHT EMISSION
• FLUORESCENCE LABELING OF PSEUDOURIDINE AND 5-FLUOROURACIL FOR HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC SEPARATION

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