機械的振動が骨芽細胞様細胞株に及ぼす影響(S04-2 医療とバイオエンジニアリング,工学技術の医療応用(2),S04 医療とバイオエンジニアリング,工学技術の医療応用)

概要

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Though mechanical stimulation is known as one of important factors promoting bone generation, the most effective frequency on it has not been specified yet. In this paper, we have investigated effects of mechanical vibration on promoting growth of MC3T3-E1, which is one of mouse calvaria osteoblast-like cell lines, and tried to specify the most effective frequency. The cells are cultured under sinusoidal vibration for 24 hours a day, 28 days at frequency of 12.5 or 100 Hz, setting an acceleration amplitude as constant of 0.5 G The cell density of the vibrating groups has been higher than that of the non-vibrating group after 14-day cultivation by counting the cells and checking their morphology through an optical microscope. The amount of generated calciumsalts in the vibrating groups is shown more than that in the non-vibrating group using alizarin red S stain solution on 28-day cultivation. It is found that the bone generation has been promoted by the mechanical vibrating stimulation.
一般社団法人日本機械学会の論文
2004-09-04