膀胱腫瘍組織内遊離基の研究 : 第2報 細胞のESR曲線の自動酸化による変化
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The clear differences of ESR spectra between normal mucosa and transitional cell carcinoma of human urinary bladder was described in the previous report of this series. In the present study the results were reconfirmed with the increased number of cases (11/13) and materials (23/25). The materials were stored in a freezer at -20℃ for a long period and the effects of spontaneous oxidization of ESR spectra were studied and the following results were obtained. (1) The degree of spontaneous oxidization was classified into the following four types by the quality and quantity of Fe^<8+> ions as the indicator. Type 1 . The absorption of Fe^<8+> is not clearly identified and the g-value is approximately 2.009 ± 0.001. Type 2. The absorption of Fe^<8+> is clear and the g-value is 2.023 ± 0.002. Type 3. The absorption of Fe^<8+> is remarkably enlarged and the g-value moves to 2.023 ± 0.007. Type 4. The absorption of Fe^<3+> disappears. Meanwhile, the effects of spontaneous oxidization on absorptions other than Fe^<8+> which is mainly hfs due to ^<14>N were analyzed. Type 1 . As mentioned in the previous report, the main difference of ESR spectra between normal mucosa and transitional cell carcinoma is that the absorption of hfs due to ^<14>N appears in the latter but not in the former. Type 2. Absorptions of ^<14>N-hfs are recognized in both normal mucosa and transitional cell carcinoma. Also the absorptions are three to five times larger than that of Type 1 . Type 3. The absorptions increase in both normal mucosa and transitional cell carcinoma or they disappear in some materials. Type 4. The ^<14>N-hfs and all other absorptions disappear. Absorptions without hfs are recognized as followings. Type 1 . The absorptions are extremely small in fresh materials while they increase thereafter by combination with oxygen. Type 2, 3 and 4. The absorption disappears with the increase of Fe^<8+>. This was seen in all materials examined but one exception.
- 社団法人日本泌尿器科学会の論文
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