腎移植の研究 : III 同種腎移植における血清蛋白の変動 : A 抗体吸収法による血清蛋白亜分画の定量法について

概要

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Quantitative measurement of serum protein subtractions by antibody absorption method was investigated for the purpose to follow up its changes in human and canine renal homotransplantation. The method is as follows : Sera for examinations are successively diluted with dilution rate H (H< 1) in a series of tubes. A certain antiserum is added to each tube and the antibody is absorbed. The supernatants of each tube are reacted against pooled sera in agar electrophoresis and the equivalent points of the respective subfraction are obtained. After the same manner the equivalent point of standard serum of known protein subfractions is determined. As protein subfraction value at the equivalent point using the same antiserum must be even, the protein subfraction value of unknown sample serum may be calculated by comparing the value of standard serum. The accuracy of protein subfraction values measured by the above mentioned method was investigated by comparing the values measured by the Fahey's method on Ig-M, Ig-G, Beta 1c/1A, Alpha -M. The correlation between the values by two methods was very close, therefore it is considered that the reliability of the value obtained by this method is very high. The serum protein fractions of 52 healthy human and 20 normal dogs were quantitatively measured by this method.
社団法人日本泌尿器科学会の論文