肝細胞と肝星細胞(<特集>肝臓領域)

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概要

• 論文の詳細を見る

• We have developed a culture system in which small hepatocytes(SHs)can grow forming colonies, when a nonparenchymal cell(NPC)fraction containing SHs and NPCs was cultured in the medium we devised(HCGM).To obtain the direct evidence that NPCs promote the growth of hepatocytes and to compare the growth potential between SHs and parenchymal hepatocytes(PHs), pure populations of SHs, PHs, and NPCs were prepared from the adult rat and co-cultures of hepatocytes and NPCs were reconstituted from them.SHs and PHs underwent multiple divisions, and the former showed a higher growth capacity than the latter when co-cultured with NPCs, whereas neither SHs nor PHs formed colonies when cultured alone.Stellate cells in the NPCs were demonstrated to be responsible for this growth promotion.We suggested that growth factors and extracellular matrices secreted by stellate cells as a result of cooperative interactions with hepatocytes stimulate the growth and differentiation of hepatocytes.

• 日本結合組織学会の論文

著者

• 立野 知世

  科学技術振興事業団・広島県地域結集型共同研究事業・広島県組織再生プロジェクト

• 立野 知世

  広島県産業科技研

• 立野 知世

  科学技術振興事業団 地域結集型共同研究事業 広島県組織再生プロジェクト

関連論文

• 1. 成体肝臓に存在する増殖性肝細胞の性質とtissue engineeringへの応用 (外科学-新しい潮流)
• 肝幹細胞と再生医学
• 肝細胞と肝星細胞(肝臓領域)

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