L-システイン残基をもつキラル担体の合成とダンシルアミノ酸のエナンシオマー分析への応用

概要

論文の詳細を見る
A chiral stationary phase containing a L-cysteine residue as a chiral selector was synthesized. 4-Chloromercuribenzoic acid was attached to a 3-aminopropylsilica-gel support by the acid-chloride activation and the carbodiimide-couling methods followed by subsequent condensation of t-butyloxycarbonyl-L-cysteine dicyclohexylamide through a free SH group. The chiral support packed in an analytical column was used for enantiomeric resolution of racemic dansylamino acids (Dns-DL-AA-OH) that was achieved by the aid of copper(II) ion added to the elution buffer. The diastereomers of the types of Dns-DL-AA-L-Trp-OMe as well as the ion-paired mixtures of Dns-DL-AA-OH and H-L-Trp-OMe (1 : 1,mol/mol) were separated on the same column without any modifiers. Hydrophobic interactions between the chiral moiety on the stationary phase and the solute complexing with copper (II) ion may mainly be involved for chiral recognition of dansylamino acid enantiomers.
福岡歯科大学学会の論文
1995-09-30