支台歯歯周組織の生化学的検索 : 高速液体クロマトグラフィーを用いた歯肉溝浸出液の蛋白分析

概要

論文の詳細を見る
Inflammatory responses in periodontal tissues cause breakdown of normal protein metabolism. The biochemical analysis of the protein components in gingival crevicular fluid (GCF) which is exudate from periodontal tissues offers a sensitive means of determining periodontal disease activity of abutment teeth. GCF proteins were applied to the high performance liquid chromatography (HPLC) analysis with following conditions : (1) column : an anion exchanger, (2) starting buffer : 20mM tris HCl pH 8.5, (3) elution buffer : 20mM tris HCl with 1 M NaCl pH 8.5, (4) gradient of Nail concentration : 0〜0.5 M=20min., 0.5〜1 M=5min., (5) flow rate : 1ml/min.. 12〜15 peaks were observed in HPLC chromatograms, they were then divided to 6 fractions and were qualitatively analysed with agarose IEF, IEP and 2 DPAGE. Determined main protein components in each peak were IgG (peak 1), transferrin and hemopexin (peak 2), C'3 and IgA (peak 3), Albumin, C'3, IgA, IgM, Haptoglobin, α_2-macroglobulin and α1-antitrypsin (peak 4), respectively. Those protein components were thought to be closely related to periodontal inflammation. It was suggested that the activity of periodontal disease was evidenced by observation of HPLC chromatogram on GCF proteins.
日本補綴歯科学会の論文
1989-06-01