鹿児島県大隅半島におけるマダニ類からの日本紅斑熱リケッチアDNAの検出およびマダニ類の季節消長

スポンサーリンク

概要

• 論文の詳細を見る

• In the present study, we attempted to detect Rlckettsia japonica DNA from ixodid ticks by the PCR technique. The seasonal occurrence of ixodid ticks was also examined in the endemic area of Japanese spotted fever. Ixodid ticks were collected at 14 sites, Futagawa (two sites), Uto, Arahira, Kamitakakuma (two sites), Onohara, Yadorihara (four sites) and Kamikawa (three sites), on the Osumi Peninsula, Kagoshima Prefecture in May and June 2001, and also collected additionally at Onohara and Kamikawa in May 2002. A total of 767 ixodid ticks was collected. They were identified as three genera and eight species as follows: Haemaphysalis flava, H. formosensis, H. longicornis, H. hystricis, Ixodes ovatus, I. nipponensis, I. turdus, and Amblyomma testudinarium. H. flava and H. longicornis seemed to be the dominant species in the survey areas. With a primer pair Rl, R2, which amplifies the genomic DNA from the spotted fever group rickettsiae, an approximately 540 bp fragment was amplified from 15 samples of six sites; H. flava (seven samples), H. longicornis (three samples), H. hystricis (four samples) and A. testudinarium (one sample). With a primer pair Rj5, RjlO, which amplifies the genomic DNA only from R. japonica, a 357 bp fragment was amplified from four samples of three sites; H. flava (three samples) and H. hystricis (one sample). H. flava seems to be the most important vector tick for Japanese spotted fever in Kagoshima Prefecture. Seasonal occurrence of the ixodid population was surveyed at three sites, Takatoge, Onohara and Komagahara from May 2002 to April 2003. H. flava nymphs were collected throughout the year, and a large number of nymphs was collected in spring. Adults showed a similar fluctuation pattern to the nymphal pattern. A large number of adults was collected from February to April but not in August and September.

• 2004-03-15

著者

• 野田 伸一

  Research Center for the Pacific Islands, Kagoshima University

• 山本 進

  Department of Hygiene, Faculty of Medicine, Kagoshima University

• NODA Shinichi

  Research Center for the Pacific Islands, Kagoshima University

• YAMAMOTO Susumu

  Department of Hygiene, Faculty of Medicine, Kagoshima University

• HONDA Toshiro

  Kagoshima Prefectural Center for Environmental Research and Public Health

• Noda Shinichi

  Research Center For The Pacific Islands Kagoshima University

• Yamamoto Susumu

  Department Of Hygiene Faculty Of Medicine Kagoshima University

• Yamamoto Susumu

  Department Of Analytical Chemistry Faculty Of Pharmaceutical Sciences Niigata University Of Pharmacy

• 本田 俊郎

  Kagoshima Prefectural Center for Environmental Research and Public Health

関連論文

• 鹿児島県大隅半島における日本紅斑熱媒介マダニ類の検索
• Parasite egg contamination of hands in a suburban area of Hanoi, Vietnam
• 鹿児島県大隅半島におけるマダニ類からの日本紅斑熱リケッチアDNAの検出およびマダニ類の季節消長
• 鹿児島県と長崎県におけるタテツツガムシの分布
• タテツツガムシ未吸着幼虫からの恙虫病リケッチアの検出と血清学的解析
• Charge and Spin Order in RNiO_3 (R=Nd, Y) by LSDA+U Method(Condensed Matter : Electronic Structure, Electrical, Mgagnetic and Optical Properties)
• The correlation between contamination of soil with Ascaris sp. eggs in school yards and ascariasis among primary school children in Mai Trung Commune, Northern Vietnam
• Parasite egg contamination of water and air in a suburban area of Hanoi, Vietnam
• Observation of Human Corneal and Scleral Collagen Fibrils by Atomic Force Microscopy
• Atomic Force Microscopy of Living Cells
• Atomic force microscopy of intact and digested collagen molecules
• The Subfibrillar Arrangement of Corneal and Scleral Collagen Fibrils as Revealed by Scanning Electron and Atomic Force Microscopy
• Imaging of Living Cultured Cells of an Epithelial Nature by Atomic Force Microscopy
• Parasite egg contamination of hands in a suburban area of Hanoi, Vietnam
• High Performance Liquid Chromatographic Determination of Acetoacetate by Post-Column Derivatization with p-Nitrobenzene Diazonium Fluoroborate
• The Effect of Specimen Diameter on Tensile Behaviors of Aluminum Thin Wires
• Multilocus Sequence Typing and DNA Similarity Analysis Implicate Borrelia valaisiana-Related sp. Isolated in Japan Is Distinguishable from European B. valaisiana

もっと見る 閉じる

スポンサーリンク