白血球貪食能の検査法について

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In order to evaluate the pathological conditions of leucocyte, it is necessary to find not only the changes of leucocyte count but also the functional conditions of leucocyte. Though there is not a satisfactory method to determine the functional activity of leucocyte, the phagocytic activity test seems to be one of the suitable methods. Many methods to determine the phagocytic activity of leucocyte have been proposed hitherto, but they are not satisfactory enough in the following respects : the facility to prepare the homogenous particle suspension, the high reproducibility of results, and the facility to observe the phagocytized particles. P-33 carbon black (Vundabilt, U.S.A.) was selected to satisfy these conditions. 150 mg of this carbon black were ground with 10 ml of 0.1% aqueous solution of gum arabic and one droplet of pure ethyl alcohol in a mortar, and centrifuged at 3000 r.p.m. for five minutes. The supernatant was recentrifuged in the same conditions. 0.1 ml of the suspension thus prepared, kept at 37℃, was added to 0.5 ml of anticoagulated blood kept in a waterbath at 37℃ and incubated for 20 minutes, being shaken for 10 seconds every five minutes. After the incubation, smears were made and stained by Field's methods. The phagocytic activity can be expressed in two ways, i.e. percentage of phagocytosing neutrophils and mean number of phagocytized particles per one neutrophil. The former was almost constant for the incubation time of 10 to 60 minutes, whereas the latter increased considerably when incubated for a long time with the suspension of high concentration. Therefore, combined with technical covenience, it seemed better to determine the percentage of phagocytosing neutrophils to know the phagocytic capacity of the whole neutrophils.
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白血球貪食能の検査法について

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