Analysis of photosystem II using particle II preparation III. Roles of cytochrome b_<559> with different redox potentials and plastocyanin in the photosynthetic electron transport system
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概要
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1. Using the P-II preparation (photosystem II preparation), the functional sites for Cyt-b^<559> with different redox potentials and for plastocyanin in the non-cyclic electron transport chain of chloroplasts were investigated. 2. In the absence of plastocyanin, the P-II preparation showed no light-induced absorption changes in Cyt-b^<559>. However, upon the addition of a sufficient amount of plastocyanin, remarkable photooxidation of this cytochrome was observed at room temperature. 3. Parallel measurements of the light-induced absorption changes in both Cyt-b^<559> and plastocyanin revealed a close relationship between them, and indicated that Cyt-b^<559> and plastocyanin are located in series on the main path of the electron transport chain involving two photoreactions in PS-II. 4. Difference spectra and action spectra for the light-induced absorption changes in Cyt-b^<559> and plastocyanin offered additional evidence in support of the above conclusion. 5. Analysis of the relationship between the activity of ferricyanide Hill reaction and the contents of Cyt-b^<559> of different redox potentials showed that both forms of Cyt-b^<559> (i.e. high- and low-potential forms) play important roles under physiological conditions, being on the main pathway of the electron transport chain connecting PS-II and PS-I. 6. To these findings it is possible to give reasonable explanations based on our scheme presented previously, which suggested that PS-II contains not one but two different photoreactions. This scheme is much more elaborately supported by this study which shows the functional sites for Cyt-b^<559> with two different potentials.
- 日本植物生理学会の論文
著者
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Huzisige Hirosi
Departrnent Of Biology Faculty Of Science Okayama University
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Huzisige Hirosi
Department Of Biology Faculty Of Science Okayama University
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Takimoto Nozomu
Departrnent of Biology, Faculty of Science, Okayama University
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Takimoto Nozomu
Departrnent Of Biology Faculty Of Science Okayama University
関連論文
- PHOTOSYNTHETIC NITRITE REDUCTASE II. FURTHER PURIFICATION AND BIOCHEMICAL PROPERTIES OF THE ENZYME
- Characteristics of Flash-Induced 515 nm Changes in Subchloroplast Preparations Highly Enriched in Photosystem II Reaction-Center Components
- Analysis of photosystem II using particle II preparation III. Roles of cytochrome b_ with different redox potentials and plastocyanin in the photosynthetic electron transport system
- Purification and properties of the photoactive particle corresponding to photosystem II
- Analysis of photosystem II using particle II preparation II. Action spectra for Hill activities and fluorescence properties of variously-treated particle II preparations