Changes in composition patterns of basic proteins in Chlorella cells during their life cycle

概要

論文の詳細を見る
(1) Changes in the composition pattern of acid soluble basic proteins in Chlorella cells during their life cycle were studied using various methods of fractionation; i. e., extraction, chromatography on CM-Sephadex column and electrophoresis on polyacrylamide gel, etc. (2) The content of basic proteins was of the order of 1-2.5% of the dry weight of cells and showed a maximum at the D~ stage and a mini-mum at the formative stages (L_2, L_3). The change was due to basic proteins (I-HCl) which were insoluble in 0.14 M NaCl and were non-elutable with 2 M NaCl from CM-Sephadex. The 0.14 M NaCl soluble basic protein content remained almost constant throughout the life cycle. (3) The I-HCl fraction in question showed 20 bands and shoulders in electrophoresis on polyacrylamide gels. Some components showed remarkable changes in their relative quantities during the life cycle, while others exhibited relatively little change. (4) Preliminary fractionation experiments of subcellular components by a non-aqueous method showed that the basic proteins showing marked changes in their relative amounts were present in a nucleus-rich fraction, and those exhibiting little change were found in a chloroplast-rich fraction, except one main component which showed characteristic behavior.
日本植物生理学会の論文