EFFECTS OF VARIOUS FACTORS ON THE INCREASE OF α-AMYLASE ACTIVITY IN RICE ENDOSPERM INDUCED BY GIBBERELLIN A_3
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概要
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The increase of α-amylase activity in embryoless rice endosperm induced by the addition of gibberellin A_3 was examined under various conditions with an aim to establish a bioassay method for gibberellins. Sterilized embryoless rice endosperms were incubated in a test tube containing 0.2-1.0 ml of test solution for 4 days at 30°. α-Amylase activity in the endosperm was determined by measuring digestion of added starch. The increase of amylase activity during the incubation was not affected by the addition of various vitamins, amino acids, organic acids, protease, sucrose, indoleacetic acid or kinetin. Helminthosporol, helminthosporic acid and sclerin (1-10 μg/ml) had weak promoting effects. Under appropriate conditions, 10^<-5> μg/ml of gibberellin A_3 could be detected. In double logarithmic plot, the increase in the enzyme activity was proportional to the gibberellin A_3 concentration in the range from 10^<-5> to 10^<-2> μg/ml.
- 日本植物生理学会の論文
著者
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Ogawa Yukiyoshi
Laboratory Applied Botany Eaculty Of Agriculture Kyoto University
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Ogawa Yukiyoshi
Laboratorium Der Angewandten Botanik Der Landwirtschaftlichen Fakultat Kyoto Universitat
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- EFFECTS OF VARIOUS FACTORS ON THE INCREASE OF α-AMYLASE ACTIVITY IN RICE ENDOSPERM INDUCED BY GIBBERELLIN A_3