PHYSIOLOGICAL AND BIOCHEMICAL CHANGES OF CARROT ROOT CALLUS DURING SUCCESSIVE CULTURES

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1. The longer the period of stock culture, the more remarkable is the growth inhibition by 8-azaguanine in callus. 2. Chloramphenicol, 5-methyltryptophane and mitomycin C exert greater inhibition on growth in CCL than in CCS. 3. Bud formation is inhibited by some concentrations of chloramphenicol without accompanying inhibition of the growth. 4. Cell size and the contents of RNA, DNA, protein and lipid per cell CCL are greater than those of CCS, respectively. The contents per cell of RNA and lipid in "mitochondrial fraction" are higher in CCL than in CCS. 5. Incorporation of guanine-8-^<14>C into RNA of CCS occurs rapidly in the first 12 hr and slows down thereafter, but that in CCL-RNA increases steadily for 16 hr. This difference in rate of the incorporation after 12 hr between CCS and CCL is principally due to the difference in rate of the incorporation into RNA of nuclear, mitochondrial and soluble fractions. The rate of RNA breakdown in CCL was not so great as the rate of synthesis. 6. 8-azaguanine (10^<-3> and 10^<-4> M) inhibits incorporation of guanine-8-^<14>C into RNA of both CCS and CCL during 14 hr, but thereafter (up to 25 hr) it inhibits the incorporation into CCL-RNA alone leaving that into CCS-RNA unaffected. In CCL 5×10^<-5>M 8-azaguanine dose not affect total radioactivity incorporated into bulk RNA, but inhibits incorporation into RNA of "mitochondrial fraction".
日本植物生理学会の論文