EFFECT OF LIPASE-DIGESTION ON PHOTOCHEMICAL ACTIVITIES OF ISOLATED CHLOROPLASTS

スポンサーリンク

概要

• 論文の詳細を見る

• In order to elucidate the role of lipids in photosynthesis, chloroplasts were digested with lipase, and the effect of lipase-digestion on some photochemical activities was studied. The HILL reaction was sensitive to the digestion, but chloroplasts having intact membrane were somewhat resistant to the action of lipase. The inactivation by lipase digestion seems to be due to the destruction of a component necessary for the HILL reaction to proceed. The chloroplasts treated with lipase showed the following activities. (1) Active photooxidation of reduced cytochrome c and menadione. (2) Photooxidation of ascorbate, which was enhanced in the presence of DPIP, and retarded in the absence of the dye. (3) NADP-photoreduction in the presence of the DPIP-ascorbate couple, as the electron donor. These facts suggested that the site attacked with lipase was responsible for the photochemical oxygen evolution. The decrease in the fluorescence intensity of chlorophyll a was also observed during the digestion.

• 日本植物生理学会の論文

著者

• Okayama Shigeki

  Department Of Biology Faculty Of Science Kyushu University:(present)biological Laboratory General Ed

• Okayama Shigeki

  Department Of Biology Faculty Of Science Kyushu University

関連論文

• Effects of Light Stress on Redox Potential Forms of Cyt b-559 in Photosystem II Membranes Depleted of Water-Oxidizing Complex : MEMBRANES AND BIOENERGETICS
• FLUORESCENCE YIELD OF CHLOROPHYLL A AND PHOTOCHEMICAL ACTIVITIES OF ISOLATED CHLOROPLASTS
• CHLOROPHYLL-SENSITIZED REDUCTION OF PLASTOQUINONE BY ASCORBIC ACID AND QUENCHING OF CHLOROPHYLL-FLUORESCENCE BY PLASTOQUINONE
• OXIDATION OF CHLOROPHYLLIN A BY CYTOCHROMF C : REMARKS ON THE ABSORPTION BAND AT 688mμ
• PHOTOOXIDATION REACTIONS BY CHLOROPLASTS SOLUBILIZED WITH SURFACE-ACTIVE AGENTS
• EFFECT OF LIPASE-DIGESTION ON PHOTOCHEMICAL ACTIVITIES OF ISOLATED CHLOROPLASTS

スポンサーリンク