PHOTOOXIDATION REACTIONS BY CHLOROPLASTS SOLUBILIZED WITH SURFACE-ACTIVE AGENTS

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概要

• 論文の詳細を見る

• 1. Solubilization of chloroplasts with a mixture of 1 per cent Duponol C and 1 per cent Span 80 (3:1) caused a destruction of activity in the HILL reaction, but the treatment brought about an increase by about 60 per cent in the rate of ascorbate photooxidation in the presence of DPIP. Heating the broken chloroplasts caused a marked decrease in the photooxidation activity. By adding surface-active agents to the boiled preparation, the activity was restored up to almost 80 per cent of the original level. 2. With colloidal suspensions of isolated chlorophylls, ascorbate was only slightly photooxidized in the presence of DPIP. By addition of the surface-active agents, the activity was greatly enhanced. 3. Dependency of the photoxidation by whole and solubilized chloroplasts and isolated chlorophyll a on the presence of DPIP was examined. DPIP can serve as an intermediate electron carrier in solubilized chloroplasts as well as in whole chloroplasts. 4. Effect of o-phenanthroline on ascorbate photooxidation by these three preparations was tested. With solubilized chloroplasts and isolated chlorophylls, the addition of the inhibitor had no influence on their ascorbate photooxidation either in the presence or absence of DPIP. 5. Treatment of whole chloroplasts with the surface-active agents induced an activity of photooxidation of cytochrome c. The electron-flow pattern for the photooxidation of ascorbate by whole and solubilized chloroplasts was briefly discussed.

• 日本植物生理学会の論文

著者

• Okayama Shigeki

  Department Of Biology Faculty Of Science Kyushu University

• Chiba Yasutane

  Department Of Biology Faculty Of Science Kyushu University

• Chiba Y.

  Department of Biology, Faculty of Science, Kyushu University

• CHIBA Y.

  Department of Biochemical Genetics Tohoku University School of Medicine

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• PHOTOPHOSPHORYLATION ACTIVITY OF CHLOROPLAST PARTICLES OBTAINED BY SONICAL TREATMENT
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