Identification of Polypeptides Associated with an Enriched Cytoskeleton-Protein Body Fraction from Developing Rice Endosperm
スポンサーリンク
概要
- 論文の詳細を見る
Recent evidence has shown that the prolamine polysomes are attached not only to the endoplasmic reticulum membranes that bound the prolamine protein bodies(PBs) but also to cytoskeleton elements associated with this subcellular fraction. To learn more about the nature of the proteins that are associated with this supra-macromolecular complex, proteins extracted from an enriched cytoskeleton-PB fraction were resolved by two-dimensional polyacryl-amid gel electrophoresis under non-equilibrium conditions and analyzed for their composition by immunological and biochemical methods. Immunoblot analysis indicated the presence of the cytoskeletal proteins, actin and tubulin, and the cytoskeletal-associated protein EF1α in this fraction. Microsequencing of selected polypeptides revealed a diversity of protein sequences. In addition to contamination storage proteins which are selectively solubilized by the isolation procedure, several ribosomal proteins and histone H3 were also identified. Some of the remaining polypeptides showed partial homology to protein sequences deposited in the database, several of which are cytoskeleton-associated proteins.
- 日本植物生理学会の論文
著者
-
OKITA Thomas
Institute of Biological Chemistry, Washington State University
-
Okita T
Washington State Univ. Washington Usa
-
Okita Thomas
Institute Of Biological Chemistry Washington State University Pullman
-
Okita Thomas
Washington State Univ. Wa Usa
-
Okita Thomas
Institute Of Biological Chemistry And Department Of Botany Washington State University
-
Hwang Y‐s
National Institute Of Agricultural Science And Technology Rda
-
Wu Yujia
Institute of Biological Chemistry, Washington State University, Pullman
-
Muench Douglas
Department of Biological Science, University of Calgary, Calgary, AB
-
Kim Yeoung-Tae
National Institute of Agricultural Science and Technology, RDA
-
Hwang Young-Soo
National Institute of Agricultural Science and Technology, RDA
-
Wu Yujia
Institute Of Biological Chemistry Washington State University Pullman
-
Kim Yeoung-tae
National Institute Of Agricultural Science And Technology Rda
-
Muench Douglas
Department Of Biological Science University Of Calgary Calgary Ab
-
Hwang Young-soo
National Institute Of Agricultural Science And Technology Rda
-
Kim Yeong-Tae
National Institute of Agricultural Science and Technology, RDA
-
Okita Thomas
Institute of Biological Chemistry
関連論文
- Genetic mechanism for transortation and accumulation of rice glutelin in protein body
- インゲンマメ ADP-glucose pyrophosphorylase cDNAs の単離と特性
- Asymmetric Localization of Seed Storage Protein RNAs to Distinct Subdomains of the Endoplasmic Reticulum in Developing Maize Endosperm Cells
- Identification of Polypeptides Associated with an Enriched Cytoskeleton-Protein Body Fraction from Developing Rice Endosperm
- Isolation and Characterization of Starch Mutants in Rice
- Transcriptional Expression Characteristics and Subcellular Localization of ADP-Glucose Pyrophosphorylase in the Oil Plant Perilla frutescens
- Molecular Cloning, Expression and Subcellular Localization of a BiP Homolog from Rice Endosperm Tissue
- Mannose Feeding and Its Effect on Starch Synthesis in Developing Potato Tuber Discs
- Interactions of the Glutelin Gt3 5' Flanking Regulatory Regions with Rice Nuclear Proteins : GROWTH AND DEVEROPMENT : GENE STRUCTURE AND EXPRESSION
- Characterization of the rice glup4 mutant suggests a role for the small GTPase Rab5 in the biosynthesis of carbon and nitrogen storage reserves in developing endosperm
- Gene-gene interactions between mutants that accumulate abnormally high amounts of proglutelin in rice seed
- Evidence for the Occurrence of Feedback Inhibition of Photosynthesis in Rice
- Protein Disulfide Isomerase Like 1-1 Participates in the Maturation of Proglutelin Within the Endoplasmic Reticulum in Rice Endosperm
- Identification of cis-Localization Elements that Target Glutelin RNAs to a Specific Subdomain of the Cortical Endoplasmic Reticulum in Rice Endosperm Cells
- Control of Starch Synthesis in Cereals : Metabolite Analysis of Transgenic Rice Expressing an Up-Regulated Cytoplasmic ADP-Glucose Pyrophosphorylase in Developing Seeds
- Metabolic Engineering of Starch for Enhanced Plant Productivity and Yields
- Accumulation of Prolamines and Glutelins during Rice Seed Development : a Quantitative Evaluation
- Expression of Storage Protein Multigene Families in Developing Rice Endosperm
- A Role for the Cysteine-Rich 10kDa Prolamin in Protein Body I Formation in Rice
- Metabolic Engineering of Starch for Enhanced Plant Productivity and Yields
- Isolation and Characterization of Starch Mutants in Rice
- Modulation of Allosteric Regulation by E38K and G101N Mutations in the Potato Tuber ADP-glucose Pyrophosphorylase