Cell-Cell Recognition during Fertilization in a Red Alga, Antithamnion sparsum (Ceramiaceae, Rhodophyta)

概要

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A gamete recognition mechanism in Antithamnion sparsum Tokida is proposed based on experiments using various lectins and carbohydrates. Spermatial binding to trichogynes is inhibited by pre-incubation of spermatia with concanavalin A (ConA) and/or L-fucose, while trichogyne receptors are blocked by the complementary carbohydrate a-methyl D-mannose and/or the lectin Ulex europaeus agglutinin (UeA1). Binding inhibition (40-50%) was observed with 10-50 mM carbohydrates and 25-50 μg ml^<-1> lectins. The inhibitory effects of ConA and UeA1 is partially reversed (to 80-90% of controls) by addition of a-methyl D-mannose and L-fucose, respectively. Lectin binding to spermatial surfaces was visualized by Fluorescein isothiocyanate (FITC) conjugated ConA, whereas carbohydrate receptors along the trichogyne and spermatium were localized with a-mannosylated-FITC-albumin and L-fucosylated-FITC-albumin, respectively. These results suggest that gamete recognition in Antithamnion sparsum is mediated by a double-docking recognition system consisting of sper-matia possessing surface L-fucose receptors and a-methyl D-mannose moiety, and trichogynes possessing the complementary receptors.
日本植物生理学会の論文