Induction of Cold Hardiness by Salt Stress Involves Synthesis of Cold- and Abscisic Acid-Responsive Proteins in Potato (Solanum commersonii Dun) : ENVIRONMENTAL AND STRESS RESPONSES

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Salt stress has been found to increase frost tolerance in some herbaceous species. In an at-tempt to understand the molecular basis of the frost tolerance induced by salt stress, the effect of salt (100 mM NaCl) on total proteins in stem-cultured potato plantlets (Solanum commersonii Dun) was analyzed on two-dimensional gels. Nine salt-induced proteins were identified after 24 h salt treatment, at which time cold hardiness increased by three degrees. Direct comparisons of the proteins with those induced by cold- and abscisic acid(ABA)-treatments revealed that five of the salt-induced proteins were also induced by cold(4°/2℃)-treatment and seven were also induced by ABA(40μM)-treatment. Three proteins (M_r/pIs 1317.0,27/6.6 and 48/6.9) were inducible in both cold- and ABA-treatments in association with frost hardening. After 6 h salt treatment, endogenous ABA levels in plantlet leaves showed a transient six-fold increase before cold hardiness developed. The results suggest that salt-induction of cold hardiness involves the synthesis of cold-and ABA-responsive proteins and the alteration of protein synthesis is mediated by ABA elevated upon salt stress. This study also suggests that a subset of proteins induced by cold- and ABA-treatments are related to salt stress.
日本植物生理学会の論文