Cytochemical Localization of Calcium and Ca^<2+>-ATPase Activity in plant Cells under Chilling Stress : a Comparative Study between the Chilling-Sensitive Maize and the Chilling-Insensitive Winter Wheat

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Electron microscopic observations revealed that electron-dense antimonate Ca^<2+> deposits were mostly localized in the vacuole and the intercellular space in both maize and winter wheat when their seedlings were grown at 25℃. The reaction products-cerium phosphate deposits of Ca^<2+>-ATPase activity were mainly seen at the cytoplasmic side of the plasma membrane. Few cerium phosphate deposits also were observed on the nuclear envelope. In both species, after 1 or 3 h 2℃ chilling, antimonate Ca^<2+> deposits increased in the cytosol and the nucleus, but cerium phosphate deposits showed no visible difference compared to their corresponding 25℃ seedlings. After 12, 24, or 72 h chilling, maize seedlings still maintained a high level of antimonate Ca^<2+> deposits in the cytosol and the nucleus. Duarig these periods, maize Ca^<2+>-ATPase, as indicated by the number of cerium phosphate deposits, became less and less active as chilling proceeded. In winter wheat, the increased cytosolic and nuclear anitimonate Ca^<2+> deposits were restored to a low resting level after 12, 24, or 72 h chilling, while the Ca^<2+>-ATPase was maintained active, contrary to maize scenario. The transient cytosolic and nuclear Ca^<2+> increase and the activities of Ca^<2+>-ATPase during chilling are discussed in relation to plant chilling injury and cold acclimation.
日本植物生理学会の論文