The β-Conglycinins of Soybean Remain Assembled in a 7S Conformation While Undergoing Proteolytic Cleavage During Germination and Early Seedling Growth : PROTEINS, ENZYMES AND METABOLISM

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概要

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• The degradation of the β-conglycinins, the second most abundant seed storage protein complex of Glycine max, that occurs as a result of proteolysis during seed germination and early seedling growth, was investigated. The β-conglycinins of soybean are composed of a semi-random association of three different subunits, a', a, and β, in a trimeric complex with a sedimentation coefficient of 7S. Western immunoblot analysis of the degradation products showed that proteolytic cleavage yields specific fragments as has been shown in other legumes. The proteolytic fragments produced in G. max, cv. Provar are designated here as FPI (62 kDa), FPII (57 kDa), FPIII (52 kDa), FPIV (31 kDa), and FPV (27 kDa). Comparison of the fragments from G. max cv. Keburi, a variant lacking the a' subunit, with those from G. max cv. Provar showed that the FPIV fragment is derived from the a' subunit. All fragments stained with periodic acid-Schiff's reagent, indicating that exhaustive deglycosylation of these subunits is not a prerequisite for cleavage. All of the fragments detected in these experiments sediment in linear sucrose density gradients with sedimentation coefficients of about 7S, identical to that of intact β-conglycinin. These results suggest that as proteolysis of the β-conglycinins occur during germination and early growth, the cleavage products are retained within the holoprotein structure. Further proteolysis cleaves the polypeptides into smaller fragments leading to the disintegration of the 7S storage protein structure.

• 日本植物生理学会の論文

著者

• Beachy Roger

  Department Of Biology Washington University:(present)department Of Cell Biology The Scripps Research

• Bryant Jerry

  Department of Biology, Washington University

• Hosangadi Poori

  Department of Biology, Washington University

• Bryant Jerry

  Department Of Biology Washington University:(current)department Of Biology University Of Missouri

• Hosangadi Poori

  Department Of Biology Washington University

関連論文

• The β-Conglycinins of Soybean Remain Assembled in a 7S Conformation While Undergoing Proteolytic Cleavage During Germination and Early Seedling Growth : PROTEINS, ENZYMES AND METABOLISM
• Accumulation of the Products of β-Conglycinin α'-Subunit Gene Constitutively Expressed in Seeds and Non-Seed Tissues of the Transgenic Petunia Plants
• Expression of Soybean Seed Storage Protein Genes in Transgenic Plants : their Effects on Expression of a Neighboring Gene and Position Dependency

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