Purification and Synthesis under Anaerobic Conditions of Rice Arginine Decarboxylase : ENVIRONMENTAL AND STRESS RESPONSES : PROTEINS, ENZYMES AND METABOLISM
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概要
- 論文の詳細を見る
Arginine decarboxylase (ADC; EC 4.1.1.19) which catalyzes the synthesis of putrescine, is involved in the responses of plants to stress. The enzyme was purified 1,561-fold from rice coleoptile by steps that included ammonium sulfate fractionation, gel filtration, ion-exchange chromatography and chromatofocusing. The purified enzyme had a pI of 5.3, a molecular mass of 176 kDa and appeared to be composed of three subunits of 63 kDa. A polyclonal antibody was raised in a rabbit and the lgG fraction was purified from serum. On Western blots the antibody recognized the ADC from both rice and E. coli. Immunoprecipitation with the ADC-specific antibodies allowed detection of radiolabelled ADC in extracts from aerobically and anaerobically grown rice seedlings that had been supplied with a mixture of ^<14>C-amino acids. This result is discussed in relation to the role of ADC under anaerobic conditions.
- 日本植物生理学会の論文
著者
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Reggiani Remo
Istitute Biosintesi Vegetali C.n.r.
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Reggiani Remo
Istituto Biosintesi Vegetali C.n.r Via Bassini
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- Purification and Synthesis under Anaerobic Conditions of Rice Arginine Decarboxylase : ENVIRONMENTAL AND STRESS RESPONSES : PROTEINS, ENZYMES AND METABOLISM
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