Proteins Associated with Specific Host-Pathogen Relationships in Infections with Wheat Rust

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The specific relationship between host and pathogen was studied using a criss-cross genetic system of well characterized, near isogenic mutants of Triticum aestivum that differed in their compatibility reaction to two genetically related mutant clones of Puccinia recondita tritici. Specific and non-specific pathogenesis-related changes in proteins were followed by pulse-labelling with [^<35>S]methionine of rust-inoculated and mock-inoculated wheat leaves, combined with SDS-PAGE and subsequent fluorography. Detectable changes in the patterns of the polypeptides synthesized "de novo" were noted as early as 3 days after infection. Synthesis of a 39-kDa polypeptide was enhanced in incompatible reactions while synthesis of an 85-kDa and a 15-kDa polypeptide were enhanced in compatible reactions. Synthesis of polypeptides present in the mock-inoculated leaves was suppressed in both the compatible (52-kDa band) and incompatible interaction systems (37-kDa band). Comparisons with a third mutant clone of rust, which elicits an inverse criss-cross relationship with the same wheat lines, indicated that most of the detected changes are likely to be associated with gene-for-gene relationships.