Subcellular localization of glucosyl transferases involved in lectin glucosylation in Pisum sativum seedlings

概要

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Cellular membranes from 4 to 5-days old etiolated pea seedlings were isolated by isopycnic centrifugation. Marker enzymes were used to measure the purity of the subcellular fractions. The formation of dolichyl-P-glucose and of glucosylated lectin was tested in all the fractions. Both activities were associated with the endoplasmic reticulum fractions. When the membranes were prepared in the presence of EDTA, a shift in both activities to a lower density in the gradient was observed. These results are in agreement with the current assumption that glycosylation of proteins via lipid-linked sugars is a cotranslational process.
日本植物生理学会の論文