Immunological Study of Carbonic Anhydrase in C_3 and C_4 Plants Using Antibodies to Maize Cytosolic and Spinach Chloroplastic Carbonic Anhydrase

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概要

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• Carbonic anhydrase (CA) was purified to homogeneity from maize leaves, and antibodies to the purified protein raised using rabbits. Western blot analyses using antibodies to the purified maize leaf CA indicated cross-reactivity with leaf extracts from a variety of C_3 and C_4 monocotyledonous and dicotyledonous plants. However maize leaf CA antibodies quantitatively titrated CA activity only in leaf extracts of maize and sorghum. Pretitration of crude leaf extracts with maize CA antibodies and activity measurements, together with Western blot analyses of the resultant samples, indicated that there are isozymic forms of CA in plant leaf tissues in both C_3 and C_4 plants which are similar in size but which differ antigenically from the maize leaf CA.

• 日本植物生理学会の論文

著者

• Burnell James

  Centre For Plant Biotechnology School Of Science And Technology Bond University

関連論文

• 高等植物のカルボニックアンヒドラーゼ
• Immunological Study of Carbonic Anhydrase in C_3 and C_4 Plants Using Antibodies to Maize Cytosolic and Spinach Chloroplastic Carbonic Anhydrase
• A Comparative Study of the Cold-Sensitivity of Pyruvate,P_1 Dikinase in Flaveria Species

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