Folylpolyglutamate Derivatives of Pisum sativum L. Determination of Polyglutamate Chain Lengths by High Performance Liquid Chromatography Following Conversion to p-aminobenzoylpolyglutamates
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概要
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The folypolyglutamate derivatives of pea seedlings (Pisum sativum L. cv. Homesteader) were extracted in the presence of 2-mercaptoethanol and cleaved to p-aminobenzoylpoly-glutamates by treatment with Zn-HCl. Azo dyes were formed by reaction with naphthylethylene diamine and purified by polyacrylamide gel chromatography. p-Aminobenzoylpolyglutamates were regenerated from these dyes by Zn treatment and then concentrated in vacuo. These derivatives were separated according to glutamyl chain length by high performance liquid chromatography on Whatman Partisil SAX columns. The folylpolyglutamates of 4 day old pea cotyledons, pea leaves and isolated chloroplasts were mainly tetra- and pentaglutamates. These and folates of shorter chain length were labelled when seeds and aerial shoots were incubated with p-aminobenzoate-[^<14^gt;C]. Labelling of the pentaglutamate was reduced in seeds that were imbibed in the presence of 0.1 mM methotrexate. Studies of cotyledon folylpolyglutamate synthetase showed that polyglutamate chain length was affected by incubation time and the concentration of tetrahydrofolate monoglutamate in the reaction system.
- 日本植物生理学会の論文
著者
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Cossins Edwin
Departmen T Of Botany University Of Alberta
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Zheng Li-li
Departmen T Of Botany University Of Alberta
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Imeson Helena
Departmen t of Botany, University of Alberta
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Imeson Helena
Departmen T Of Botany University Of Alberta
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