Mutations in β-Tubulin Block Transhyphal Migration of Nuclei in Dikaryosis in the Homobasidiomycete Coprinus cinereus

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β-Tubulins from fourteen benomyl-resistant strains of the homobasidiomycete Coprinus cinereus, which carry the benA, benB, benC or benD mutations, were analyzed by urea SDS-PAGE or isoelectric focusing and subsequent immunoblot analysis. Electrophoretic aberrations in a major β-tubulin isotype, denoted β1, were found in two strains, BEN154 and BEN215, both of which carry benomyl resistance mutations in benA^+. The aberrations of β1 in BEN154 and BEN215 cosegregated with benomyl resistance among the progeny of outcrosses of BEN154 and BEN215 to wild type, indicating that the β1 aberrations were caused by the benA mutations. Both the mutant and wild-type β1 tubulins were present in the heterozygous dikaryons, BEN154/wild-type and BEN215/wild-type, ruling out post-translational modification as a possible cause for the aberrations in β1. Thus,we conclude that benA is a structural gene for β1. Transhyphal migration of nuclei in dikaryosis was blocked in the mycelia of BEN154 and in its progeny that carried benA (β1 mutation), demonstrating that microtubules are involved in the migration process. Nuclear migration in dikaryosis seems to differ in terms of mechanism, at least in part, from the migration of tetrad nuclei from basidia into prespores during formation of basidiospores and from the migration of nuclei from basidiospores into hyphae during germination, because a benA mutation blocked the former without affecting the latter two processes.
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Mutations in β-Tubulin Block Transhyphal Migration of Nuclei in Dikaryosis in the Homobasidiomycete Coprinus cinereus

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