Regulation of NADH-Glutamate Dehydrogenase Activity by Phytochrome, Calcium and Calmodulin in Zea mays

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概要

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• Regulation by the active form of phytochrome (P_<FR> and the effect of Ca^<2+> and calmodulin was examined with glutamate dehydrogenase (GDH) of Zea mays. A brief irradiation (5 min) to 5 day old plants with red light resulted in 5-6 fold increase in GDH activity. This effect was nullified when red light was followed immediately by far-red light. The photoreversibility showed that P_<FR> regulates GDH activity in vivo. To the enzyme extract obtained after EGTA treatment, when Ca^<2+> was added in vitro, GDH was activated by 6 fold. The maximum response by Ca^<2+> was obtained at 80μM. Both P_<FR> and Ca^<2+> effects were found to be age dependent. The enzyme activity was inhibited by compound 48/80 in partially purified extracts and the effect was reversed by calmodulin. The purified GDH, however, was not activated directly by calmodulin; it required the presence of another protein factor which was separated by gel permeation column by HPLC. Neither anticalmodulin drugs nor addition of calmodulin had any effect on nitrate reductase activity.

• 日本植物生理学会の論文

著者

• Sharma Arun

  Plant Research Laboratory School Of Llfe Sciences Jawaharlal Nehru University

• Sharma Arun

  Plant Research Laboratory School Of Life Sciences Jawaharlal Nehru University

• Sopory S

  Jawaharlal Nehru Univ. New Delhi Ind

• Sopory Sudhir

  Plant Molecular Biology Group International Centre For Genetic Engineering And Biotechnology

• Sopory Sudhir

  Plant Research Laboratory School Of Llfe Sciences Jawaharlal Nehru University

• Das Rina

  Plant Research Laboratory, School of Llfe Sciences, Jawaharlal Nehru University

• Das Rina

  Plant Research Laboratory School Of Llfe Sciences Jawaharlal Nehru University

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• Regulation of NADH-Glutamate Dehydrogenase Activity by Phytochrome, Calcium and Calmodulin in Zea mays
• Phytochrome Control of Acid and Alkaline Phosphatases in Etiolated Shoot Apices of Sorghum bicolor
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