HYDROGENASE REACTIONS IN RHODOPSEUDOMONAS PALUSTRIS

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概要

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• 1. The hydrogenase reactions in a purple non-sulfur bacterium Rhodopseudomonas palustris, were investigated. Under photoheterotrophic culturing conditions, the photosynthetic activity of the cells was found to be closely paralleled by the activity of hydrogenase. It was also revealed that the bacterium can grow under such conditions even withont both photosynthetic and the enzyme activities. 2. The enzyme was revealed to be localized in the particulate fraction (presumably chromatophores) of the disintegrated cells. The properties of the enzyme in the cell-free preparation and in intact cells were described. 3. Among various hydrogen acceptors tested, p-benzoquinone was most rapidly hydrogenated. Some heat-labile factor was shown to be involved in the reduction of quinine, which was not required in the reduction of methylene blue. 4. The reactions of hydrogenase, both in cell-free state (quinine- and MB-reduction and in intact cells (oxyhydrogen reaction), were markedly inhibited by molecular oxygen. The inhibition was noncompetitive with respect to H_2. A reversible mole-to-mole combination of the enzyme and O_2 was suggested as the mechanism of the inhibition. 5. Carbon monoxide inhibition was suggested also to be caused by a reversible mole-to-mole combination of the enzyme and the inhibitor. This inhibition was competitive with respect to H_2. 6. Rhodopseudomonas palustris hydrogenase was found to be refractory toward cyanide, azide and sulfhydryl reagents. 7. Light markedly suppressed the oxyhydrogen reaction (intact cells) whereas other hydrogenation reactions (intact cells and cell-free preparations) were not affected by illumination.

著者

• Izawa Seikichi

  Department Of Biophysics And Biochemistry Faculty Of Science University Of Tokyo:(present)laboratory

• Izawa Seikichi

  Department Of Biophysics And Biochemistry Faculty Of Science University Of Tokyo

• Izawa S.

  Department Of Mechanical Engineering Tohoku University

関連論文

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