Plant Cell Wall Proteins : Partial Characterization of Maize Wall Proteins With Putative Roles in Auxin-Induced Growth

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Antibodies raised against cell wall proteins inhibited auxin-induced growth of Zea mays L. coleoptile segments. The total complement of proteins isolated from the cell walls of Zea mays seedlings was fractionated by cation exchange and gel filtration chromatography. A procedure was developed to evaluate these cell wall-protein fractions for their ability to reverse growth inhibition cause by specific antibody binding. Inhibition of growth was attributed to specific antibody-antigen interaction based on the observations that only serum containing antibodies against certain cell wall proteins inhibited growth, that gamma globulins purified from appropriate serum samples inhibited growth, and that a specific subfraction of isolated cell wall proteins precipitate the growth inhibiting antibody. Antigens which generated growth inhibitory antibodies were identified as an acidic group of proteins with apparent relative molecular masses in the range of 20-25 kDa. This subfraction of cell wall proteins was not effective in hydrolyzing cell wall polysaccharides. A small amount of carbohydrate was found associated with this fraction and may reflect some degree of glycosylation of some of the proteins.
日本植物生理学会の論文