Characterization of the Hydrolytic Activity of Avocado Cellulase

概要

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The cellulase produced by ripening avocado fruits (Persea americana Mill cv. Fuerte) was isolated and purified using chromatofocusing (pH 7-4) and gel filtration on a Bio-Gel P-100 column. Characteristics of the cellulase were assessed by using, as substrates, a range of polysaccharides containing various sugar residues and varying types of linkages between the residues. Only those substrates containing (1→4)-β-glucosyl linkages were hydrolyzed by the purified enzyme. Two polysaccharides that were extensively hydrolyzed by the cellulase were carboxymethylcellulose and (1→3),(1→4)-β-D-glucans such as from Avena endosperm cell walls. Characterization of the activity in the degradation of the mixed linked glucan of Avena and cellodextrins indicated that the enzyme has a limit recognition-hydrolytic site of four (1→4)-β linked glucose residues. It was also found that the enzyme could cleave only (1→4)-β-linkages that were adjacent to other (1→4)-β-D-glucosyl linkages. Activity of the cellulase against isolated avocado fruit cell walls indicated that the purified enzyme was incapable of appreciably solu-bilizing the cellulosic components of these walls.
日本植物生理学会の論文