Localization of Mannose, N-Acetylglucosamine and Galactose in the Golgi Apparatus, Plasma Membranes and Cell Walls of Scenedesmus acuminatus

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The localization of lectin binding sites in the Golgi apparatus, plasma membranes and cell walls of Scenedesmus acuminatus was investigated by cytochemical electron microscopy. The lectins used were concanavalin A (Con A), peanut agglutinin (PNA) and wheat germ agglutinin (WGA), all labeled with gold. Con A-gold particles were deposited not on the Golgi apparatus, but on the outer cell-wall layer. PNA-gold and WGA-gold particles were deposited on distal Golgi cisternae and vesicles derived from the Golgi apparatus. Entire cell-wall layers were evenly labeled by PNA-gold. The plasma membrane and cytoplasmic regions close to the plasma membrane were labeled with WGA-gold. The processing of oligosaccharide in the Golgi apparatus, plasma membranes and cell walls of Scenedesmus acuminatus is discussed in reference to that reported for animal cells.
日本植物生理学会の論文