Photoinhibition of glucose Uptake in Chlorella

概要

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In colorless mutant cells of Chlorella vulgaris (M125), endogenous respiration in the dark was not affected by 30-min preillumination with white light (9,000 mW・m^<-2>), while exogenous respiration of glucose or fructose was inhibited significantly by the same treatment in air, but not under N_2. This light effect on exogenous respiration was accompanied by an inhibition of hexose uptake. When autotrophically grown wild-type cells of Chlorella vulgaris (211-11 h) were incubated in glucose medium with DCMU, light also greatly inhibited glucose uptake and growth. Blue light was very effective, while red light had only a slight effect. This photoinhibitory effect was also observed in algal cells that had been grown in a glucose-containing medium in the dark. Using SDS-gel electrophoresis, a new protein peak with a molecular weight of 35-40 kDa was detected in plasma membrane-rich cell wall fractions when Chlorella vulgaris (211-11 h) cells were transferred to a glucose-containing medium. This peak disappeared after the algal cells were returned to the glucose-free medium. These findings suggest that this protein includes the hexose-carrier protein. Blue light significantly inhibited the formation of this protein during incubation in a glucose-containing medium.
日本植物生理学会の論文