ATP-Promoted Sorbitol Transport into Vacuoles Isolated from Apple Fruit
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概要
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Sorbitol was transported actively into vacuoles isolated from apple (Malus pumilla Mill. var domestica Schneid.) fruit flesh. The uptake was stimulated up to twofold by the addition of ATP, and the ATP dependent uptake showed a saturation curve as to the substrate concentration. The optimum uptake of sorbitol was pursued in the acidic range of pH 5 to 6. The K_m value for the ATP dependent sorbitol uptake was about 5 mM. Sorbitol uptake was clearly inhibited by PCMB and uncouplers (CCCP and DCCD), and to a lesser extent by orthovanadate, but only slightly by oligomycin. K^+ stimulated sorbitol uptake. Sorbitol was converted to other sugars (glucose) only very slowly when transported across the tonoplast. This suggests that sorbitol is transported into vacuoles by a carrier mediated transport system coupled with H^+-ATPase, localized on the tonoplast. Sucrose uptake into the vacuoles was also enhanced by ATP.
- 日本植物生理学会の論文
著者
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Yamaki Shohei
Fruit Tree Research Station Ministry Of Agriculture Forestry And Fisheries:(present)laboratory Of Ho
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Yamaki Shohei
Fruit Tree Research Station (ministry Of Agriculture Forestry And Fisheries)
関連論文
- Localization of Sorbitol Oxidase in Vacuoles and Other Subcellular Organelles in Apple Cotyledons
- Isolation of Vacuoles from Immature Apple Fruit Flesh and Compartmentation of Sugars, Organic Acids, Phenolic Compounds and Amino Acids
- Energy Coupled Transport of Sorbitol and Other Sugars into the Protoplast Isolated from Apple Fruit Flesh
- NADP^+-Dependent Sorbitol Dehydrogenase Found in Apple Leaves
- ATP-Promoted Sorbitol Transport into Vacuoles Isolated from Apple Fruit
- Distribution of Sorbitol, Neutral Sugars, Free Amino Acids, Malic Acid and Some Hydrolytic Enzymes in Vacuoles of Apple Cotyledons
- A sorbitol oxidase that converts sorbitol to glucose in apple leaf
- Subcellular Localization of Sorbitol-6-phosphate Dehydrogenase in Protoplast from Apple Cotyledons