An Accurate Transcription of Wheat Histone Genes in Sunflower Cells

概要

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Wheat histone H3 and H4 genes were introduced into the nuclear genome of sunflower by Ti plasmid-mediated gene transfer. Three to 10 copies of the genes were stably integrated into the genome. The introduced genes were transcribed in the transformed sunflower cells. S1 analysis of the transcript of the introduced gene indicated that the 5' and 3' ends of their transcripts were mapped on the sites identical to those of the transcripts of the same genes in wheat seedlings. The measurement of the relative amount of the transcripts showed that the quantity of the H3 transcript was about 7 times more than that of the H4 transcript in the transformed cells. This value was comparable with that observed in wheat seedlings, suggesting that the wheat H3 and H4 genes introduced in sunflower genome were transcribed in the same regulatory manner as done in wheat cells.
日本植物生理学会の論文