Interactions between the Nucleus and Cytoplasmic Organelles during the Cell Cycle of Euglena gracilis in Synchronized Cultues : II. Associations between the Nucleus and Chloroplasts at an Early Stage of the Cell Cycle under Photoautotrophic Conditions

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Chloroplast-nucleus interactions were examined in cells of Euglena gracilis Z synchronized under photoautotrophic conditions. The chloroplasts were localized near the cell periphery. At an early stage of the cell cycle, however, some chloroplasts were transiently located in the inner space close to the nucleus. Electron microscopy using serial cell sections revealed that the chloroplast formed protrusions at several sites, which became associated with the nucleus. The outer membrane of the chloroplast envelope was in contact, or at least continuous in part, with the outer membrane of the nuclear envelope at the sites of association, and dense material was present in the chloroplast membrane. A chromosome was close to each site of the association between these two organelles. Most of the chloroplasts including those in association with the nucleus were connected by fine bridges. The 4',6-diamidino-2-phenylindole-stained nucleoids in the chloroplast associated with the nucleus appeared to have a thread-like shape. There was another type of chloroplast-nucleus connection, in which an intervening membranous body was in contact with the outer part of the nuclear envelope on one side and with the chloroplast envelope on the other side.
日本植物生理学会の論文