Crown Gall Tumorigenesis in the Forage Legume Medicago sativa L.
スポンサーリンク
概要
- 論文の詳細を見る
Seedlings of the forage legume Medicago saliva formed crown gall tumours at low frequency following inoculation with octopine and nopaline strains of Agrobacterium tumefaciens. There occurred a plant variety-bactcrial strain specificity with respect to tumorigenesis, some Medicago varieties failing to develop tumours following inoculation. In comparison, all bacterial strains tested incited tumours on stem explants of Nicotiana tabacum cv. Xanthi. DNA-DNA hybridization revealed a simple T-DNA pattern in an uncloned and cloned Medicago tumour incited by the octopine strain ACH5, protoplasts cloning indicating the T-DNA copy number to be about 5 copies per genome. Comparison of the T-DNA of octopine positive and octopine negative B6 tumours showed a correlation between absence of part of the T-DNA and loss of opine synthesis. Although non-transformed tissues of Medicago readily regenerate plants, this ability was suppressed in transformed tissues.
- 日本植物生理学会の論文
著者
-
Freeman J.p.
Plant Genetic Manipulation Group Department Of Botany University Of Nottingham
-
Draper J.
Plant Genetic Manipulation Group, Department of Botany, University of Nottingham
-
Davey M.R.
Plant Genetic Manipulation Group, Department of Botany, University of Nottingham
-
Cocking E.C.
Plant Genetic Manipulation Group, Department of Botany, University of Nottingham
-
Mariotti D.
Plant Genetic Manipulation Group, Department of Botany, University of Nottingham
-
Draper J.
Plant Genetic Manipulation Group Department Of Botany University Of Nottingham
-
Davey M.r.
Plant Genetic Manipulation Group Department Of Botany University Of Nottingham
-
Mariotti D.
Plant Genetic Manipulation Group Department Of Botany University Of Nottingham:(present)centro Migli
-
Cocking E.c.
Plant Genetic Manipulation Group Department Of Botany University Of Nottingham
関連論文
- A Comparison of Methods for Plasmid Delivery into Plant Protoplasts
- Crown Gall Tumorigenesis in the Forage Legume Medicago sativa L.
- Ti Plasmid Homologous Sequences Present in Tissues from Agrobacterium Plasmid-transformed Petunia Protoplasts