Ti Plasmid Homologous Sequences Present in Tissues from Agrobacterium Plasmid-transformed Petunia Protoplasts

スポンサーリンク

概要

• 論文の詳細を見る

• Suspension cell protoplasts of albino Petunia hybrida have been transformed by isolated Agrobacterium tumefaciens Ti plasmid. Uptake of octopine Ti plasmid (pTiACH5) into protoplasts was stimulated by poly-L-ornithine and polythylene glycol (PEG). The frequency and efficiency of transformation of protoplasts to phytohormone autotrophy was compared using the two uptake agents with various concentrations of plasmid. Transformation was most efficient with PEG-mediated uptake, 5μg of Ti plasmid per 10^6 protoplasts giving a frequency of 6×10^<-5>. Octopine was not synthesised in any of the transformants after the second subculture on hormone-free medium. DNA-DNA hybridisation showed the presence of DNA homologous to the T-DNA region of pTiACH5 in all clones analysed.

• 日本植物生理学会の論文

著者

• Freeman J.p.

  Plant Genetic Manipulation Group Department Of Botany University Of Nottingham

• Draper J.

  Plant Genetic Manipulation Group, Department of Botany, University of Nottingham

• Davey M.R.

  Plant Genetic Manipulation Group, Department of Botany, University of Nottingham

• Cocking E.C.

  Plant Genetic Manipulation Group, Department of Botany, University of Nottingham

• Cox B.J.

  Bio Sciences Group, ICI Corporate Laboratory

• Cox B.j.

  Bio Sciences Group Ici Corporate Laboratory

• Draper J.

  Plant Genetic Manipulation Group Department Of Botany University Of Nottingham

• Davey M.r.

  Plant Genetic Manipulation Group Department Of Botany University Of Nottingham

• Cocking E.c.

  Plant Genetic Manipulation Group Department Of Botany University Of Nottingham

関連論文

• A Comparison of Methods for Plasmid Delivery into Plant Protoplasts
• Crown Gall Tumorigenesis in the Forage Legume Medicago sativa L.
• Ti Plasmid Homologous Sequences Present in Tissues from Agrobacterium Plasmid-transformed Petunia Protoplasts

スポンサーリンク