Cyanide-Insensitive Respiration of Isolated Cells

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The respiration of isolated tobacco leaf cells and the effects of cyanide and salicylhydroxamic acid (SHAM) on respiration were analysed. The S/V versus S plot showed the existence of two Km(O_2) with an oxygen concentration ranging between 1 μM and 11 μM Cyanide remarkably depressed the respiratory rate and increased Km(O_2) values immediately after cell isolation. The effects of cyanide were reduced by incubation of cells under light. According to results of kinetic studies, the cyanide-sensitive oxidase in intact cells is considered to be a Cyt oxidase and the cyanide-insensitive oxidase to be the alternate oxidase. Cyt oxidase was inhibited competitively by cyanide (K_i=7.5 × 10^<-9> M) and the alternate oxidase was inhibited, also competitively, by SHAM (K_i=1.3 × 10^<-5> M) in vivo.
日本植物生理学会の論文