Stabilization and Transport Capacity of Cowpea and Barley Vacuoles
スポンサーリンク
概要
- 論文の詳細を見る
Subjecting either cowpea or barley protoplasts to a combined osmotic and pH shock provides the optimum conditions for the isolation of cowpea and barley vacuoles. Incubation of vacuoles in a defined medium resulted in 50% lysis after 30 min (cowpea) and 20 min (barley). The addition of 1 mM EDTA resulted in increased stability of vacuoles with 50% lysis occurring after 50 min (cowpea) and 120 min (barley). Other compounds were tested for their effects on the stability of vacuoles. The longer life of vacuoles in the presence of EDTA allowed transport studies to be carried out using radiolabeled tracers. The uptake of [^<14>C]sucrose (10 mM) by cowpea vacuoles was stimulated approximately two-fold by the presence of MgATP (10 mM) ; the Km for [^<14>C]sucrose uptake by cowpea vacuoles was 12.5 mM. Uptake of [^3H]GA_1 ([^3H]gibberellin A_1) by cowpea vacuoles was also stimulated two-to-four fold in the presence of 10 mM MgATP compared to untreated vacuoles. No MgATP stimulation of [^3H]GA_1 or [^<14>C]sucrose uptake could be observed in barley vacuoles. The effect of pH on uptake of [^3H]GA_1 was studied in both cowpea and barley vacuoles. Uptake was optimal at about neutral pH which also coincided with the optimum pH for maximum stability of vacuoles.
- 日本植物生理学会の論文
著者
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Knuth Mark
Department Of Vegetable Crops-plant Growth Laboratory University Of California At Davis:(present)int
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Garcia-martinez Jose
Department Of Vegetable Crops-plant Growth Laboratory University Of California At Davis:(present)ins
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Rappaport Lawrence
Department Of Vegetable Crops University Of California
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Rappaport Lawrence
Department Of Vegetable Crops-plant Growth Laboratory University Of California At Davis
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Keith Brian
Department of Vegetable Crops-Plant Growth Laboratory, University of California at Davis
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Clark Craig
Department of Vegetable Crops-Plant Growth Laboratory, University of California at Davis
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Clark Craig
Department Of Vegetable Crops-plant Growth Laboratory University Of California At Davis
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Keith Brian
Department Of Vegetable Crops-plant Growth Laboratory University Of California At Davis
関連論文
- Stabilization and Transport Capacity of Cowpea and Barley Vacuoles
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- REGULATION OF BUD REST IN TUBERS OF POTATO SOLANUM TUBEROSUM L. II. INHIBITION OF SPROUTING BY INHIBITOR β COMPLEX AND REVERSAL BY GIBBERELLIN A_3
- Abscisic acid and the accumulation, biological activity and metabolism of four derivatives of [^3H] gibberellin A_1 in barley aleurone layers
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