Assimilation of ^<15>NH_3 by Root Nodules Detached from Soybean Plants
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概要
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The assimilation of ^<15>NH_3 by crude breis prepared from crushed soybean nodules was examined. The highest enrichment during 60 min of reaction time with ^<15>N was found in alanine and the next highest in the amide-N of asparagine and glutamate. The labelling of allantoic acid was relatively low, although it was higher than that of other amino compounds. Nodule breis were separated into a bacteroid fraction and a supernatant plant fraction, and the ^<15>NH_3 incorporation into the main nitrogen compounds by each fraction was determined. The bacteroid fraction was much more efficient in converting ^<15>NH_3 into glutamate, alanine and glycine than the supernatant fraction, while for allantoic acid, the supernatant fraction showed a greater ability. The incorporation of ^<15>NH_3 into allantoic acid was strongly inhibited by the addition of azaserine or allopurinol, and enhanced by organic acid compounds, especially fumarate, succinate and malate. The mode of ureide formation in the course of ammonia assimilation in the soybean nodule is discussed.
- 日本植物生理学会の論文
著者
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Yamaguchi Masuro
Department Of Agricultural Chemistry College Of Agricullure University Of Osaka
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Fujihara Shinsuke
Department of Agricultural Chemistry, College of Agricullure, University of Osaka
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Fujihara Shinsuke
Department Of Agricultural Chemistry College Of Agricullure University Of Osaka
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Yamaguchi Masuro
Department o f Agricultural Chemistry, College o f Agriculture, University
関連論文
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