Mass Propagation of Begonia × hiemalis Plantlets by Shake Culture

概要

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The conditions for rapid growth of Begonia plantlets from buds regenerated on leaf segments were studied, the ultimate aim being to develop a mass propagation procedure. Plantlet growth was stimulated in submerged culture by Murashige-Skoog's medium with 10 or 30 g/liter sucrose, but was inhibited by a lower (×1/4) concentration of basal medium. Root development was superior in the lower strength medium. The greater amount of sucrose, 30 g/liter, stimulated root growth more than the 10 g/liter did. Buds required kinetin at a concentration of 0.3 to 1 mg/liter, but did not require auxin. Plantlets reached maximum growth after 3 weeks of cultivation in 300-ml Erlenmeyer flasks containing 30 to 150 ml of medium that were shaken at 180 rpm. Cultivation in jar and bubble column fermentors appears to be advantageous for the large scale propagation of Begonia plantlets.
日本植物生理学会の論文