Mineral nutrition of cultured chlorophyllous cells of tobacco VII. : Effects of the NH_4/NO_3 ratio and Cl in the media on the growth and ionic balance of cells

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NG, a strain of cultured tobacco cells of Nicotiana glutinosa had high growth rates and carboxylate contents (C-A) of 100 to 130 meq/100 g of dry cells on media containing 42 meq NO_3/liter as the sole N source. (C-A) is the amount of inorganic cations minus inorganic anions in meq per 100 g of dry cells. NG, cultured on media containing NH_4 10 + NO_3 42 in meq/liter, had lower growth rates and lower (C-A) values as compared with NG on media containing NO_3 as the sole N source. NG, cultured on media containing NH_4 30 + NO_3 42 in meq/liter, had high growth rates and (A-C) values of 22 to 53 meq/100 g of dry cells. In this case, the (A-C) content may correspond to organic cations, basic organic N compounds such as free and protein-bound basic amino acids. The easily absorbed Cl may have been required to maintain good growth conditions such as ionic balance and a favorable pH in the cells. Thus cultured cells of Nicotiana glutinosa may have physiological adaptability against variations in a relatively wide range of |C-A| contents [|C-A| being the absolute values of (C-A)].
日本植物生理学会の論文