Analysis of RNase isozymes in gelminating pea cotyledons by polyacrylamid(Fgel electrophoresis

概要

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In germinating pea cotyledons, at least three different RNase-isozymes can be detected by column chromatography (6). Polyacrylamide gel electrophoresis analysis in the present study revealed the presence of five bands, with at least two being associated with heavily stained protein bands in reference gels. Assays carried out in the presence of the nuclease inhibitor diethylpyrocarbonate (DEPC) showed that these bands were most likely artificial and not ribonucleases (RNases). The nature of a fast-running component with the staining behavior of RNase, which neither can be inhibited by DEPC nor is associated with a heavily stained protein band, is discussed. It may be a small molecule in the range of 20,000 daltons. This band was also present in the cotyledons of dry seeds. When transfer RNA was used as a substrate in the assay, the band with the lowest mobility degraded this substrate very rapidly.
日本植物生理学会の論文