Evidence for the involvement of cytokinins in the regulation of protcolytic activity in cotyledons of germinating beans

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The proteolytic activity of an extract of cotyledons of Phaseolus vulgaris increased during the first 7 days of germination, both when casein was the substrate and when the endogenous protein served as such. The increase was partially dependent on the presence of the embryo axis. The effect of the axis was replaceable by kinetin or zeatin, but not by gibberellic acid or IAA. During the period of 3-6 days after the beginning of incubation, the level of amino acids in cotyledons attached to the embryo axis was lower than in detached ones. No such difference could be detected in the first 2 days of incubation. On the other hand, the influence of the axis on protease activity already was clearly detectable on the second day of germination. Application of casein hydrolysate to the seeds brought about an increase in the concentration of amino acids in the cotyledons, but no simultaneous de-crease in the protease level could be detected. We concluded that these results do not confirm the hypothesis that the influence of the axis on proteolytic activity is due to its function as a sink for amino acids produced in the cotyledons.
日本植物生理学会の論文