Use of inhibitors to distinguish between C_4 acid decarboxylation mechanisms in bundle sheath cells of C_4 plants

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概要

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• Both malate and aspartate were clecarboxylated at the 4-carbon position by isolated bundle sheath strands of C_4 plants but to different extents depending upon the species. In Digitaria sanguinalis, an NADP-malic enzyme (NADP-ME) species, 100 μM oxalic acid blocked malate decarboxylation through NADP-ME without affecting aspartate decarboxylation which apparently occurs through NAD-ME. In several phosphoenolpyruvate carboxykinase (PEP-CK) type C_4 species, 200 μM 3-mercaptopicolinic acid (3-MPA), an inhibitor of PEP-CK, specifically inhibited the malate decarboxylation and partially inhibited aspartate decarboxylation. The aspartate decarboxylation insensitive to 3-MPA may occur through NAD-ME. Neither inhibitor prevented C_4 acid decarboxylation in bundle sheath cells of NAD-ME species. The inhibitors thus served to diffrentiate between the decarboxylation of C_4 acids in PEP-CK and NADP-ME type C_4 species through their major decarboxylase from that of their less active decarboxylation through NAD-ME.

• 日本植物生理学会の論文

著者

• Edwards G.e.

  Department Of Botany Washington State University:institute Of Biological Chemistry Washington State

• Rathnam C.k.m.

  Department Of Horticulture University Of Wisconsin

• Edwards G.E.

  Department of Horticulture, University of Wisconsin

関連論文

• Distribution of enzymes related to C_3 and C_4 pathway of photosynthesis between mesophyll and bundle sheath cells of Panicum hians and Panicum milioides
• Photosynthetic Characteristics of C_3-C_4 Intermediate Flaveria Species II. : Kinetic Properties of Phosphoenolpyruvate Carboxylase from C_3-C_4 and C_3-C_4 Intermediate Species
• Differences in cold lability of pyruvate,Pi dikinase among C_4 species
• Photosynthetic efficiency of Panicum hians and Panicum milioides in relation to C_3 and C_4 plants
• Glycolate synthesis in a C_3, C_4 and intermediate photosynthetic plant type
• Use of inhibitors to distinguish between C_4 acid decarboxylation mechanisms in bundle sheath cells of C_4 plants

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