Chromatographic behaviour of phytochrome following its in vivo phototransformation

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Soluble phytochrome of maize coleoptiles does not readily permeate Sephadex G200 and is eluted in the void volume as a single species. The exclusion by Sephadex G200 is not due to molecular aggregation. This property of maize phytochrome is found with material extracted from both the dark grown or far red and red irradiated tissues; and at 4℃ elution proceeds under normal laboratory lighting as it does in darkness or under a green safelight. During chromatography on Sepharose 4B of soluble extracts of maize coleoptiles containing soluble phytochrome it behaves as a single species and it is eluted at a position similar to that of catalase. Phytochrome recovered from the phytochrome-binding site complex which is formed inside cells of coleoptiles following irradiation with red light is eluted ahead of catalase suggesting that it has gained in size. A further size increase (about 2-fold) is observed when the particulate fraction containing the complex' is treated with Triton X100. Control experiments demonstrate that the size increase is due neither to molecular aggregation nor to gross protein-denaturation but is probably due to a change in molecular con-figuration.
日本植物生理学会の論文