Auxin-gibberellin relationships in their effects on hypocotyl elongation of light-grown cucumber seedlings IV. Inhibition of IAA-induced elongation by N,N'-dicyclohexylcarbodiimide and its reversal by gibberellin A_3

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IAA-induced elongation and control growth of light-grown cucumber hypocotyl sections were markedly inhibited by DCCD, an inhibitor of membrane-bound ATPases. The concentration effective for inducing marked inhibition was more than 10^<-5> M. At 10^<-5> M DCCD, there was an apparent antagonism between IAA and DCCD. At 5 × 10^<-5> M DCCD, the inhibition was partially recovered by 10^<-4> M of IAA. The results might indicate a close association of the auxin action with membrane-bound ATPases. The DCCD inhibition was so strong that treatment with 10^<-4> M DCCD for about 5 min significantly suppressed further growth and longer incubation killed the sections. In contrast, DCCD had not inhibitory effect on both control growth and IAA-induced elongation if GA_3 was present simultaneously. DCCD treatment followed by GA_3 treatment still resulted in the inhibition, suggesting that the inhibition was not eversible. In order to obtain reversal of DCCD inhibition by GA_3 both compounds must be present at the same time. The GA_3 effect is discussed in connection with the mechanism of DCCD action on membrane-bound ATPases.
日本植物生理学会の論文