Ragi malt, a growth factor, for Vigna sinensis Endl. callus tissues

概要

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Callus tissue obtained from hypocotyl segments of Vigna sinensis was capable of indefinite growth on modified White's medium supplemented with I mg/liter each of 2,4-D and kenitin, but its rate of growth was slow. Ragi (Eleucine coracana L.) malt extract obtained from the 'IE-810' variety promoted a 38 and 30-fold increase in the fresh and dry weights, respectively, over the controls (modified White's with 2,4-D and kinetin). Similarly 100% 'Aruna' ragi malt extract elicited a nearly 40-fold increase in both the fresh and dry weights, which were as high as 21.1 g (fresh weight) and 1.64 g (dry weight) over the controls (0.48 for fresh and 0.04 g for dry weight). Growth values of 183 and 1 63, in respect to the fresh and dry weights were obtained in a period of 5 weeks. Analysis of 'Aruna' ragi malt extract showed the presence of 17 amino acids. Totally 72% (Sugars 30, myo-inositol 10, amino acids 20 and inorganic fractions 12) of the growth promoting activity of ragi malt extract was accounted for. Ragi malt extract also exhibited cytokinin-like activity when assayed with Vigna callus which has an absolute requirement for a cytokinin and auxin.
日本植物生理学会の論文