The development of structure and function in chloroplasts of greening mutants of Scenedesmus I. Formation of chlorophyll

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概要

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• Several mutant strains of Scenedesmus obliquus have been isolated which show only traces of chlorophyll when grown heterotrophically but, when transferred to light, develop chlorophyll in amounts equivalent to the wild-type strain. The action spectrum for greening of one of these mutants, C-2A', while in a stationary growth phase, revealed a predominant influence of blue light with a maximum effectiveness around 460 nm; possible involvement of either carotenoids or flavoproteins in the greening response is suggested. The greening process is absolutely dependent upon light and oxygen and the kinetics demonstrate two distinctive phases. The initial phase is blue-light dependent and apparently linked energetically to the "respiratory" capacity of the cell, whereas the second one depends upon the increased photosynthetic capacity resulting from the initial stage of chlorophyll production. Chlorophyll synthesis ceased completely when cultures were transferred to the dark but recommensed at the same rate when once again placed in the light. The onset of greening is accompanied by an enhancement of respiration and of polysaccharide utilization. The rate of greening of C-2A' is markedly influenced by temperature in the range of 20 to 30℃. Greening is prevented by anaerobic conditions, potassium cyanide, dinitrophenol, sodium arsenite, sodium fluoroacetate and sodium azide. Inhibition of the second phase also results when photosynthesis is prevented, i.e., by DCMU inhibition or carbon dioxide exclusion. Possible mechanisms for greening in this mutant are discussed.

• 日本植物生理学会の論文

著者

• Senger Horst

  Botanisches Institut Der Universitat Marburg

• Senger Horst

  Botanisches Institut Der Universitat Marburg-lahn Germany And Department Of Botany Oregon State Univ

• Bishop Norman

  Botanisches Institut der Universitat Marburg-Lahn, Germany and Department of Botany, Oregon State Un

• Bishop Norman

  Botanisches Institut Der Universitat Marburg-lahn Germany And Department Of Botany Oregon State Univ

関連論文

• The development of structure and function in chloroplasts of greening mutants of Scenedesmus III. Biosynthesis of δ-aminolevulinic acid
• Spectral effectiveness in chlorophyll and 5-aminolevulinic acid formation during regreening of glucose-bleached cells of Chlorella protothecoides
• The development of structure and function in chloroplasts of greening mutants of Scenedesmus I. Formation of chlorophyll
• Quantum yield and conformational changes during greening and bleaching of Chlorella protothecoides
• The development of structure and function in chloroplasts of greening mutants of Scenedesmus IV. Blue light-dependent carbohydrate and protein metabolism
• Studies on nucleic acids in plastids of the pigment mutant C-2A' of Scenedesmus obliquus

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